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Cell apoptosis FITC-Annexin V apoptosis detection kit I (Beyotime, Shanghai, China) was used to determine the effect of OA-NPs@OA on HaCaT cell apoptosis induction using flow cytometry

Hepatic low-density lipoprotein receptor concentration and hepatic stearoyl-CoA desaturase 1 gene expression were determined, in addition to histopathological studies of heart and liver tissues

There are many warning signs of potential vitamin B2 deficiency in the pregnant mothers, including i) Gestational Diabetes - lower functional B2 leads to poorer processing of blood glucose, leading to elevated blood sugar ii) Elevated TSH - Insufficient Iodine in the mothers leads to an increase in TSH levels during pregnancy, leading to hypothyroidism iii) Low T3 - Insufficient Selenium in the mothers - either due to increased demand, or low intake, leads to lower T3 in the mother iv) Difficulty in getting pregnant

Representative tissue specimens were embedded in paraffin, sectioned at 4 m, stained with hematoxylin and eosin (H&E), and evaluated by light microscopy using an Olympus 71 digital camera and an Olympus BX51 microscope (Japan) acquiring digital images saved as uncompressed 24-bit RGB TIFF files