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To follow up these investigations, we next examined the capacity of the individual serine doublet mutant receptors to elicit downstream signalling responses in INS-1 832/3 GLP-1R KO cells, including analyses of GLP-1-induced cAMP generation and -arrestin 2 recruitment to the receptor

The LR3 modification provides a 20-30 hour half-life (vs minutes for native IGF-1), reduced IGFBP binding, and approximately 2-3x potency

After four weeks, the dose increases to 5 mg weekly

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