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For each primer set, both the annealing temperature and the number of cycles were optimized (Supplementary Data 2, 3, 6)

Zinc ions (Zn) not only play an important role in mucosal repair and anti-inflammatory processes in intestinal damage but can also complex with EGCG through coordination, thereby enhancing EGCGs stability and maintaining its activity [21,22,23,24,25,26,27,28]

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Both the common effect model (8.83) and the random effects model (8.87) have odds ratios (OR) that show a statistically significant increase in vomiting in the treatment group