For 24 h experiments, a 10 ml culture (2.5% hematocrit, 34% parasitemia) of ring stage 3D7 or Dd2 strains expressing hGrx1-roGFP2 were treated with antimalarial drugs at 4IC 50 for 24 h
Additionally, Qiu et al
Tryptone Soy Broth (TSB) was obtained from Oxoid (Thermo Fisher Scientific, Australia), Enzymes: DNase-I was obtained from Invitrogen (Melbourne, Australia), -Amylase from MP Biomedicals (NSW, Australia) and Proteinase K from Sigma-Aldrich (Sydney, Australia)
However, oxidative stress or GSH depletion causes TXNIP to dissociate from TXN and instead bind to NLRP3, inducing conformational activation and promoting inflammasome assembly 53,54

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