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To confirm the phenotype changes were driven by OGDHc inhibition, we also treated cells with DM-2OG, mimicking impaired 2-OG metabolism by the OGDHc, and found that, similarly to ML226, DM-2OG increased the CCR7 high CD45RO high population, with a corresponding decrease in the CCR7 low CD45RO high population (Fig

Mammalian cell culture, transfection and generation of stable cell lines Mammalian cell cultures were performed following previously published protocols 6

doi:10.1177/1076029613481103 Zhang Y, Shi H, Wang W, et al

From these results, we conclude that the deSUMOylase activity is not essential for autoproteolytic processing of SnCE1

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Geng N, Shi B-J, Li S-L, Zhong Z-Y, Li Y-C, Xua W-L, et al