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In total, these analyses comprised 94 biological or technical replicates including control and treated samples, 6 extraction blank injections used for background subtraction, 7 injections of a quality control plasma sample, 42 injections of representative pools used for the generation of peak lists (targets) and lipid annotations based on MS/MS

The separation via HPLC follows an isocratic method at 30 C using a reversed-phase column in two runs

Modeling nanocarrier transport across a 3D in vitro human bloodbrain-barrier microvasculature