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Cells were resuspended in 1 ml of KPBS, 10l of cells were transferred into 40ml of 1% Triton lysis buffer for a whole-cell protein sample and 10l of cells were transferred into 50ml of 80% methanol, containing heavy labeled amino acid standards, for direct extraction of whole-cell metabolites

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In rats, more severe effects were seen after maternal exposure to semaglutide in the form of visceral abnormalities and major skeletal malformations ( Table 1 )