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Cell lysis and protein extraction For protein extraction, cells were disrupted with a cell scraper in a lysis buffer consisting of 50 mM HEPES-Na, pH 7.2, 0.5 M NaCl, 0.5% (v/v) NP-40, 0.05% LDS and 1Pierce EDTA-free protease inhibitor cocktail (Thermo, Australia) in Milli-Q H 2 O and then vortexed

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Smanjenje teine je bilo vee kod gojaznih mnukaraca koji su imali BMI vei od 35 u poreenju sa gojaznim mukarcima koji su imali BMI manji od 35, a isto se odnosilo i na ene